Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours
B, Z., El-Sharabassay, A., Dwidar, Y., Ashour, A., Ahmed, E., Arafat, M. (2017). Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours. EKB Journal Management System, 63(3), 89-98. doi: 10.21608/vmjg.2017.355070
Zeidan B; A El-Sharabassay; Y Dwidar; A Ashour; E Ahmed; M Arafat. "Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours". EKB Journal Management System, 63, 3, 2017, 89-98. doi: 10.21608/vmjg.2017.355070
B, Z., El-Sharabassay, A., Dwidar, Y., Ashour, A., Ahmed, E., Arafat, M. (2017). 'Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours', EKB Journal Management System, 63(3), pp. 89-98. doi: 10.21608/vmjg.2017.355070
B, Z., El-Sharabassay, A., Dwidar, Y., Ashour, A., Ahmed, E., Arafat, M. Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours. EKB Journal Management System, 2017; 63(3): 89-98. doi: 10.21608/vmjg.2017.355070

Influence of Different extenders on semen quality of the male’s dromedary camel during incubation at 37 oC for up to 6 hours

Veterinary Medical Journal (Giza)
Volume 63, Issue 3, July 2017, Page 89-98  XML PDF (5.69 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2017.355070
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Authors
Zeidan B1; A El-Sharabassay2; Y Dwidar3; A Ashour2; E Ahmed1; M Arafat* 2
1Animal Production Research Institute, Dokki, Giza, Egypt
2Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
3Depaartment of Biotechnology, Faculty of Agriculture, Al-Azhar, University, Cairo, Egypt
Abstract
fifteen male dromedary camels at 5-10 years of age and 500-600 kg live body weighs were used the present study, semen was collected, evaluated and extended with different six extenders (Glucose- yolk- citrate: GYC, Fructose- yolk – citrate: FYC, Lactose -yolk – citrate: LYC, sucrose – Yolk: SYC, Tris-Yolk-Fructose: TYF and Raffinose yolk citrate: RYC) thus final extension rate was 1 semen:4 extender citrate. The extended semen with different extenders (TYP, GYC, PYC, SYC, LYC and RYC) was incubated at 37oC for up to 6 hours. After each incubation time (0 ,1, 2, 4, 5 and 6 hours) the spermatozoa and sperm motility, dead spermatozoa, abnormal spermatozoa and acrosomal damage of spermatozoa where determined sperm penetration into she-camel cervical mucus during incubation at 37oC for up to 4 hours was also estimated. The results revealed that, the extended camel semen with TYF, LYC or SYC or SYC extenders was significantly (P<0.05) higher the percentage of sperm motility, while significantly (P<0.05) lower the percentage of dead spermatozoa abnormal spermatozoa and acrosomal damage of spermatozoa than GYC, FYC and RYC extenders during incubation at 37oC for up to 6 hours. The advancement of incubation time at 37°C decreased significantly (P<0.05) the percentage of motile camel spermatozoa, while significantly (P<0.01) increased the percentage of dead spermatozoa, abnormal spermatozoa and acrosomal damage of spermatozoa with all different extenders (GYC, FYC SYC.LYC, TYP and RYC). The Penetrating ability of the extended camel spermatozoa into she-camel Cervical mucus increased significantly with TYP or LYC extender compared to other extenders during incubation at 37 °C for up to 4 hours. However, the advancement of incubation time at 37oC decreased significantly (P<0.05) the ability to penetrate cervical mucous of camel spermatozoa of the all used extenders.
Keywords
Dromedary camels; semen; extenders; incubation; penetration
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