Comparison of Antimicrobial Resistance in Escherichia coli Strains Isolated from Diseased Broiler and Cattle based on Phenotypic and Genotypic Analysis | ||||
| Egyptian Journal of Animal Health | ||||
| Volume 4, Issue 2, April 2024, Page 268-279 PDF (536.8 K) | ||||
| Document Type: Original researches | ||||
| DOI: 10.21608/ejah.2024.355806 | ||||
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| Abstract | ||||
| Escherichia coli infections in poultry and animals have become a global problem. Currently, little is known regarding the epidemiology of extended-spectrum beta-lactamases (ESBLs) producing Escherichia coli in infected poultry and cattle. This study aimed to identify antibiotic resistance patterns and ESBL-producing genes in E. coli strains recovered from diseased poultry and animals in northern Egypt. A total of 100 consecutive, no duplicate clinical samples from diseased broiler poultry and 50 samples from diseased cattle were collected during a one-year period. ESBL was found in 44% (24 out of 54) isolates of E. coli isolated from infected poultry positive for double disc diffusion, and 66% (16 out of 24) isolates of E. coli recovered from cattle positive for double disc diffusion. ESBL genes (blaCTX-M1, blaCTX-M2, blaOXA10, blaSHV, blaTEM, blaOXA-2, blaVEB, blaPER-2, and blaGES) were detected in strains by multiplex polymerase chain reaction with gene-specific primers. From 40 phenotypically positive ESBL isolates, we discovered that blush, blaTEM, blactx-m1, and blactx-m9 (100%) were the most prevalent genotypes in cattle isolates, whereas blaGes and blactx-m9 (70% of isolates from poultry). This is the first study to look at ESBL resistance patterns and ESBL-producing genes in E. coli isolated from poultry and cattle. Our analysis found varying occurrences among isolates from various hosts. | ||||
| Keywords | ||||
| beta-lactamases (ESBLs) producing; Escherichia coli; Antibiotic-resistant; PCR detection | ||||
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