Acid Tolerance and Growth of Four Strains of Bifidobacteria in Different Types of Milk | ||||
| Journal of Food and Dairy Sciences | ||||
| Article 5, Volume 9, Issue 7, July 2018, Page 251-255 PDF (424.16 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jfds.2018.36014 | ||||
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| Authors | ||||
| Raghda M. S. Moawad1; Sabah T. Abdel-Razek1; A. S. Zahran1; F. S. Ibrahim2 | ||||
| 1Dairy Department , Faculty of Agriculture , Minia university. | ||||
| 2Dairy Department , Faculty of Agriculture , Minia university | ||||
| Abstract | ||||
| Four strains of bifidobacteria ( Bifidobacterium angulatum ATCC 2238, Bifidobacterium animalis subsp lactis Bb12 , Bifidobacterium bifidum ATCC 2203, and Bifidobacterium bifidum LMG 10645) were studied for acid tolerance and growth in different types of mammalian milks . All studied strains grew at a wide range of pH , ranging from pH 2.0 - pH 6.5 . However the growth was quite low at pH 2.0 , it was in the range between (0.07 - 0.12 optical density at 660 nm ) and slightly higher when the pH of the medium was raised to pH 3.0 , at this pH the growth was between ( 0.08 - 0.18) optical density at 660 nm . On the other hand , at pH 4.0 the optical density was ranging between ( 0.90 - 2.10 at 660 nm) . The higher growth rate was achieved at pH 6.5 , it was between (3.55- 4.35) optical density at 660 nm. The studied strains were grown in human, cow, goat, buffalo, sheep and camel milks. All strains were able to grow at the six types of milk , the growth was in the range between 1.5 x106 cfu /ml (sheep milk ) - 2.1x107 cfu /ml (cow milk ) a slight decrease was noticed when Bifidobacterium angulatum 2238 was grown in sheep milk . Bifidobacterium bifidum 2203 reached the count of 10 x109 cfu /ml in goat milk and the lowest growth was noticed in human milk . The slowest growth of Bifidobacterium animalis subsp lactis Bb12 was 7.5 x105 cfu /ml , it was found when it was grown in camel milk and the best growth was noticed in goat and sheep milks . Bifidobacterium bifidum LMG 10645 grew well in all types of milk except human milk , the growth rate was almost around 106 - 107 cfu /ml,whereas a slight decrease was noticed when they grew in human milk 5.9 x106 cfu /ml. | ||||
| Keywords | ||||
| Bifidobacteria; acid tolerance; cow; Goat; buffalo and camel milks | ||||
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