Administration of olive oil optimizes acetic acid induced colitis in CD1 mice
Ali, D., Salem, M., Tousson, E. (2018). Administration of olive oil optimizes acetic acid induced colitis in CD1 mice. EKB Journal Management System, 2(2), 1-8. doi: 10.21608/jcbr.2018.37763
Dina Abd El Aziz Ali; Mohamed Salem; Ehab M Tousson. "Administration of olive oil optimizes acetic acid induced colitis in CD1 mice". EKB Journal Management System, 2, 2, 2018, 1-8. doi: 10.21608/jcbr.2018.37763
Ali, D., Salem, M., Tousson, E. (2018). 'Administration of olive oil optimizes acetic acid induced colitis in CD1 mice', EKB Journal Management System, 2(2), pp. 1-8. doi: 10.21608/jcbr.2018.37763
Ali, D., Salem, M., Tousson, E. Administration of olive oil optimizes acetic acid induced colitis in CD1 mice. EKB Journal Management System, 2018; 2(2): 1-8. doi: 10.21608/jcbr.2018.37763

Administration of olive oil optimizes acetic acid induced colitis in CD1 mice

Egyptian Journal of Cancer and Biomedical Research
Article 2, Volume 2, Issue 2 - Serial Number 3, August 2018, Page 1-8  XML PDF (534.4 K)
Document Type: Original Article
DOI: 10.21608/jcbr.2018.37763
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Authors
Dina Abd El Aziz Ali email 1; Mohamed Salemorcid 2; Ehab M Tousson3
1Department of Zoology, Faculty of Science, Tanta University, Egypt.
2Immunology and Biotechnology Division, Zoology Department, Faculty of Science, Tanta University, Egypt. Center of Excellence in Cancer Research, Tanta Teaching Hospitals.
3Zoology Department, Faculty of science, Tanta University, Egypt.
Abstract
Colitis is a chronic disease that affects individuals at different ages and affects the quality of their life.  So far, there are three different experimental models for colitis. However, animals in these models die quickly duo to the associates toxicity. The aim of this study is to optimize acetic acid induced acute colitis mouse model with olive oil co-administration. Male albino CD1 mice (n= 91) were randomly divided into 13 groups (n= 7/group). To induce colitis, mice were administrated with different volumes (100 or 200 µl) and concentrations (1%, 2% and 4%) of acetic acid by intrarectal (IR) injection either for three consecutive days or every other day for a total of 6 days. Olive oil (1 µL) was co-injected with acetic acid.  One day later, mice were sacrificed and colon tissues were fixed in 10% formalin for histological examinations. The induction of colitis by acetic acid was dependent on both the volume and the concentration of acetic acid. Interestingly, co-administration of olive oil with different volumes and concentrations of acetic acid, although did not alter the severity of inflammation, it increased the animal survival rate and decreased the associated toxicity.  Injection of co-administration of olive oil with acetic acid is a new modification to enhance the application of this model.
Keywords
acetic acid; colitis; Colon; model
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