ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES
Afify, A., Hauka, F., Kassem, M., El-Sawah, A. (2015). ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES. EKB Journal Management System, 6(10), 435-445. doi: 10.21608/jacb.2015.48443
Aida H. Afify; F.I.A. Hauka; M.M. Kassem; A.M. El-Sawah. "ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES". EKB Journal Management System, 6, 10, 2015, 435-445. doi: 10.21608/jacb.2015.48443
Afify, A., Hauka, F., Kassem, M., El-Sawah, A. (2015). 'ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES', EKB Journal Management System, 6(10), pp. 435-445. doi: 10.21608/jacb.2015.48443
Afify, A., Hauka, F., Kassem, M., El-Sawah, A. ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES. EKB Journal Management System, 2015; 6(10): 435-445. doi: 10.21608/jacb.2015.48443

ENHANCEMENT OF INULINASE PRODUCTION FROM AN Acetobacter sp. IN SUBMERGED CULTURES

Journal of Agricultural Chemistry and Biotechnology
Article 4, Volume 6, Issue 10, October 2015, Page 435-445  XML PDF (336.43 K)
Document Type: Original Article
DOI: 10.21608/jacb.2015.48443
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Authors
Aida H. Afify; F.I.A. Hauka; M.M. Kassem; A.M. El-Sawah
Microbiol. Dept., Fac. Agric., Mansoura Univ., Egypt.
Abstract
Recently, inulinases one of the most important enzymes which have gained much attention in industrial purposes. They can be applied for production of high fructose syrup from inulin and could reduce the process steps, resulting in cost reduction and increasing the amount of products. Acetobacter sp. produced extracellular inulinase  in vitro. Maximum inulinase production was obtained at pH 7.0, 300C temperature, ammonium sulphate as nitrogen source, inoculum volume 5% (v/v), 1% sucrose as additional carbon source and 48 hr after inoculation.The enzyme showed maximum activity at pH 7 and 50 0C. The enzyme was stable more than 80% of its maximal activity at 30-50 0C after 30 min heat treatment.
Keywords
Acetobacter sp; inulinase; inulin; hydrolysis; high fructose syrup
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