Assessment of irradiated Staphylococcus aureus isolates as a vaccine
M. Gamal, I., M. El-Sonbaty, S., Mahmoud, H., A. Alam, H., S. Abdelkhalek, N. (2018). Assessment of irradiated Staphylococcus aureus isolates as a vaccine. EKB Journal Management System, 35(2), 12-30. doi: 10.21608/bvmj.2018.48552
Inas M. Gamal; Sawsan M. El-Sonbaty; Hala Mahmoud; Hanaa A. Alam; Noha S. Abdelkhalek. "Assessment of irradiated Staphylococcus aureus isolates as a vaccine". EKB Journal Management System, 35, 2, 2018, 12-30. doi: 10.21608/bvmj.2018.48552
M. Gamal, I., M. El-Sonbaty, S., Mahmoud, H., A. Alam, H., S. Abdelkhalek, N. (2018). 'Assessment of irradiated Staphylococcus aureus isolates as a vaccine', EKB Journal Management System, 35(2), pp. 12-30. doi: 10.21608/bvmj.2018.48552
M. Gamal, I., M. El-Sonbaty, S., Mahmoud, H., A. Alam, H., S. Abdelkhalek, N. Assessment of irradiated Staphylococcus aureus isolates as a vaccine. EKB Journal Management System, 2018; 35(2): 12-30. doi: 10.21608/bvmj.2018.48552

Assessment of irradiated Staphylococcus aureus isolates as a vaccine

Benha Veterinary Medical Journal
Article 2, Volume 35, Issue 2, December 2018, Page 12-30  XML PDF (690.99 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.48552
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Authors
Inas M. Gamal1; Sawsan M. El-Sonbaty2; Hala Mahmoud3; Hanaa A. Alam4; Noha S. Abdelkhalek5
1Immunity unit-Animal production research institute-5 mrkz bhoth alhdaik street-Alharam-Giza
2Department of Radiation microbiology, The National center for radiation research and technology (NCRRT), Atomic Energy Authority. P. O. Box: 29 Nasr City, Cairo, Egypt
3Central laboratory for Evaluation of veterinary Biologics- alseka albeda-Abasia- Cairo-Egypt.
4Department of mastitis and neonatal disease- Animal production research institute-5 mrkz bhoth alhdaik street-Alharam-Giza.
5Department of reproduction diseases-Animal production research institute-5 mrkz bhoth alhdaik street-Alharam-Giza.
Abstract
Studies were initiated in an attempt to produce an inactivated vaccine of high potency with low toxicity. In this study structural integrity of bacteria can be preserved when different radiation doses of gamma rays were used to inactivate S. aureus. The results revealed that the 7 KGy of γ- radiation was the most suitable dose for attenuation S. aureus. Moreover, this study demonstrated that exposure of S. aureus to γ- radiation at a dose of 7 KGy inactivated bacterial cells growth with low DNA damage and did not alter bacteria protein profile. Meanwhile, higher radiation doses caused an obvious DNA damage and alteration in the bacterial protein profile. Furthermore, the effect of gamma irradiated S. aureus on the innate and adaptive immune components after vaccination was demonstrated. All the measured immunological parameters (TLR2, IL10, IL1β, iNOs, TGFβ, MAPK and PI3K) were increased when compared to the control group. This approach could expedite vaccine production for emerging and established pathogens for which no protective vaccines exist. 
Keywords
Staphylococcus aureus; vaccine; gamma radiation
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