Omega-3 polyunsaturated fatty acids as therapeutic options for the treatment of ethanol induced gastric ulcer in rats | ||||
| Benha Veterinary Medical Journal | ||||
| Article 6, Volume 34, Issue 1, March 2018, Page 48-65 PDF (347.12 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2018.53523 | ||||
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| Authors | ||||
| Yakout El Senosi; Mohamed Mahfouz; Alaa Hussein | ||||
| Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt. | ||||
| Abstract | ||||
| Gastric ulcer is a common chronic disease in human digestive system. Massive alcohol drinking can lead to gastric ulcer. In the present study we investigated the gastroprotective effect and the molecular mechanisms of Omega 3 polyunsaturated fatty acids(ώ-3 PUFAs) in a rat model of ethanol-induced gastric ulcer.42 male rats were divided into six equal groups. Group 1 :( Control normal group) rats received no drugs. Group 2 :( Omega-3 group): rats receivedOmega-3 orally(244.8 mg/kg b.wt/day) for 14 days. Group 3 :( early ulcer group):rats received absolute Ethanol (0.5ml/rat) orallyand sacrificed one hour later after ethanol administration Group 4: (Early ulcer + Omega-3 protected group) rats received omega-3 similar to group 2 for 14 days prior to absolute ethanol administration, then sacrificed after one hour. Group5: (late ulcer group) rats received absolute ethanol similar to group 3 and sacrificed after 14 days. Group6:( Late ulcer + Omega-3 treated group)rats first administered with absolute ethanol then after one hour omega-3 was administered for 14 days. The results showed significant increase in No,L-MDA,GSH with marked decrease in CAT activity in stomachof ethanol- induced gastric ulcer in rats as compared with control normal group.However, a significant depletion of gastric tissue No, L-MDA and GSH concentrations and marked increase in CAT activity were observed after omega-3 treatment compared to ulcerated untreated rats. The qPCR results revealed a significant up-regulation of mRNA gene expression levels of TNF-a, MOP and iNOS in stomach of ethanol-induced gastric ulcer in rats. This expression was significantly down-regulatedafter administration of omega 3.Conclusively, omega-3 protects rat gastric mucosa against ethanol-induced gastric ulcer via anti-inflammatory and anti-oxidative mechanisms. | ||||
| Keywords | ||||
| omega-3; ethanol, gastric ulcer; Oxidative Stress; Anti-inflammatory | ||||
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