Phenotypic and genotypic characterization of listeria species isolated from chicken and milk products
Abd El Tawab, A., Hassan, W., El- Hofy, F., Zaki, H. (2018). Phenotypic and genotypic characterization of listeria species isolated from chicken and milk products. EKB Journal Management System, 34(1), 94-105. doi: 10.21608/bvmj.2018.53531
Ashraf Abd El Tawab; Wafaa Hassan; Fatma El- Hofy; Haidy Zaki. "Phenotypic and genotypic characterization of listeria species isolated from chicken and milk products". EKB Journal Management System, 34, 1, 2018, 94-105. doi: 10.21608/bvmj.2018.53531
Abd El Tawab, A., Hassan, W., El- Hofy, F., Zaki, H. (2018). 'Phenotypic and genotypic characterization of listeria species isolated from chicken and milk products', EKB Journal Management System, 34(1), pp. 94-105. doi: 10.21608/bvmj.2018.53531
Abd El Tawab, A., Hassan, W., El- Hofy, F., Zaki, H. Phenotypic and genotypic characterization of listeria species isolated from chicken and milk products. EKB Journal Management System, 2018; 34(1): 94-105. doi: 10.21608/bvmj.2018.53531

Phenotypic and genotypic characterization of listeria species isolated from chicken and milk products

Benha Veterinary Medical Journal
Article 9, Volume 34, Issue 1, March 2018, Page 94-105  XML PDF (428.03 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.53531
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Authors
Ashraf Abd El Tawab1; Wafaa Hassan2; Fatma El- Hofy3; Haidy Zaki2
1Bacteriology,Immunology and Mycology Dep., Fac.Vet.Med.Benha Univ
2Animal Health Research
3Bacteriology,Immunology and Mycology Dep., Fac.Vet.Med.Benha Univ.
Abstract
Listeria monocytogenes has became increasingly important as a food-associated pathogen. It can cause a rare but serious disease called listeriosis with high fatality rates (20–30%) compared with other foodborne microbial pathogens. To estimate the incidence and levels of  Listeria  spp. in different food sources, A total of 200 random samples which collected from different sources 65 samples isolated from poultry , 45 from poultry byproducts (Frozen chicken meat balls (kofta), Frozen chicken burger and frozen chicken sausages), 20 from pasteurized milk ,30 from milk products (Feta cheese ,cream cheese )and 40 from poultry eggs .Only 7(14%) of samples that collected from suspected isolates and detected on Oxford agar, PALCAM agar and ALOA agar were suspected to be Listeria spp. In  poultry, poultry byproducts, pasteurized milk, milk byproducts and poultry eggs, listeria percentage were 0/65(0%), 4/45(1.8), 0/20(0%), 0/30(0%) ,3/40(1.2%)  isolates, respectively. The in-vitro antimicrobial sensitivity test showed that the isolated L.monocytogenes were sensitive to Sulphamethoxazole /trimethoprim, Gentamycin ,Ceftazidime and Cephazoline followed by Sulphamethoxazole, ceftriaxone , weak sensitivity to Penicillin .  While they were resistant to Erythromycin, Clindamycin, Ampicillin. The results of virulence tests for isolated listeria strains appeared that all of L.monocytogenes were virulent strains as all of them were positive to CAMP test; showed narrow zone of β–hemolysis on sheep blood agar .The PCR results for Listeria isolates  showed that all strains are L.monoctogenes and have Inl A and hly A genes positive as virulence genes while negative Inl B, iap and prf A genes. 
Keywords
L.monocytogenes; Poultry; Milk
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