Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine
bayoumi, M., Moustafa, A., Madkour, N., Selim, A., Abdelazez, W. (2018). Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine. EKB Journal Management System, 34(1), 106-116. doi: 10.21608/bvmj.2018.53535
Mohamed bayoumi; Abdelmoneim Moustafa; Nashwa Madkour; Abdelfattah Selim; Wafaa Abdelazez. "Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine". EKB Journal Management System, 34, 1, 2018, 106-116. doi: 10.21608/bvmj.2018.53535
bayoumi, M., Moustafa, A., Madkour, N., Selim, A., Abdelazez, W. (2018). 'Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine', EKB Journal Management System, 34(1), pp. 106-116. doi: 10.21608/bvmj.2018.53535
bayoumi, M., Moustafa, A., Madkour, N., Selim, A., Abdelazez, W. Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine. EKB Journal Management System, 2018; 34(1): 106-116. doi: 10.21608/bvmj.2018.53535

Trial for Preparation of Combined Equine Influenza virus, Equine Herpesvirus-1 and Tetanus Toxoid Vaccine

Benha Veterinary Medical Journal
Article 10, Volume 34, Issue 1, March 2018, Page 106-116  XML PDF (288.42 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.53535
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Authors
Mohamed bayoumi1; Abdelmoneim Moustafa2; Nashwa Madkour1; Abdelfattah Selimorcid 2; Wafaa Abdelazez1
1Equine Viral Disease Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia
2Animal medicine department, Faculty of Veterinary Medicine, Benha University
Abstract
Vaccination remains an important strategy to fight Equine influenza (EI), Equine herpesvirus-1 (EHV- 1) and tetanus infection, in combination with management measures. A combined inactivated EIV, EHV-1 and Tetanus toxoid (TT) vaccine adjuvanted with saponin and Alhydrogel was prepared and tested in four groups of guinea pigs in comparison with each vaccine separately beside another group as control. The humoral immune response in vaccinated guinea pigs against EI, EHV-1 and tetanus was evaluated by Haemagglutination Inhibition (HI) antibody titers, Enzyme linked immunosorbent assay (ELISA) titers, and toxin neutralizing (TN) antibody titers respectively. All vaccines proved to be safe and potent in guinea pigs. Also, the obtained antibodies titers with combined vaccine was nearly similar to titer obtained with separated vaccine of each target agent in vaccinated guinea pigs. The evaluation of keeping quality of the prepared combined inactivated vaccine showed that it is was stable at 4C up to 12 months and for 3 months at room temperature.   
Keywords
Equine influenza; herpesvirus-1; tetanus toxoid; saponin; Alhydrogel vaccine; Potency
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