The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats
Abdel-Hamid, O., Nafee, A., M.A, E., M.A, E. (2018). The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats. EKB Journal Management System, 34(1), 329-343. doi: 10.21608/bvmj.2018.54257
Omnia M Abdel-Hamid; Abeer A Nafee; Emam M.A; Elshimaa M.A. "The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats". EKB Journal Management System, 34, 1, 2018, 329-343. doi: 10.21608/bvmj.2018.54257
Abdel-Hamid, O., Nafee, A., M.A, E., M.A, E. (2018). 'The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats', EKB Journal Management System, 34(1), pp. 329-343. doi: 10.21608/bvmj.2018.54257
Abdel-Hamid, O., Nafee, A., M.A, E., M.A, E. The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats. EKB Journal Management System, 2018; 34(1): 329-343. doi: 10.21608/bvmj.2018.54257

The ameliorative effect of Vitamin C in experimentally induced colon cancer in rats

Benha Veterinary Medical Journal
Article 30, Volume 34, Issue 1, March 2018, Page 329-343  XML PDF (916.41 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.54257
View on SCiNiTO View on SCiNiTO
Authors
Omnia M Abdel-Hamid1; Abeer A Nafee2; Emam M.A3; Elshimaa M.A4
1Department of Biochemistry, Faculty of Veterinary Medicine, Benha University
2Department of Physiology, Faculty of Veterinary Medicine, Benha University
3Department of Histology, Faculty of Veterinary Medicine, Benha University
4Department of Biochemistry, Faculty of Science, Ainshams University
Abstract
The present study was designed to investigate the ameliorative effect of vitamin C administration on serum  alanine aminotransferase (ALT),aspartate aminotransferase(AST),Urea, Creatinine, Alphafetoprotein(AFP), Carcinoembryonic antigen (CEA) and carbohydrate antigen 19.9 (CA 19- 9), in addition to tumor necrosis factor alpha (TNF-α) , Cytochromep4502E1 and Caspase-9 gene expression in colon cancer induced by 1,2-dimethylhydrazine (DMH)in rats. Forty white female albino rats were divided into four equal groups (10 each). Group I(control group):received no drugs. Group II:(DMH group): rats injected subcutaneously with DMH (35 mg/kg body weight),twice a week for 5 consecutive weeks for colon cancer induction. Group III:(protective  group): rats administrated vit C(200 mg kg-1 b. wt) orally and for 4 weeks before DMH injection and continued with vit C administration daily till the end of the experiment .Group IV:(treatment group):rats injected subcutaneously with DMH then administrated Vit C for 6 weeks till the end of the experiment. Blood and colon tissue samples were collected from all animal groups at the end of the experiment. The obtained results showed that DMH injection to rats significantly increased serum(ALT, AST, Urea, Creatinine, AFP, CEA, CA 19-9) and colon tissue TNF-α and CYP2E1while colon tissue caspase-9 gene wassignificantly decreasedwhen compared to control.Administration of Vit C significantly decreased serum (ALT, AST, Urea, Creatinine, AFP, CEA, CA 19-9) in addition to TNF-α and  CYP2E1in colon tissue. How every, caspase-9 gene expression showed a significant increase. Histopathological examination of colon tissue edesquamation of the colon epithelium with heavy leukocytic infiltrationin DMH group. While administration of vitamin C in colon cancer induced rats showed mild destruction of the lining epithelium with mild leukocytic infiltration in addition to mild malignancy of epithelium when compared to DMH group. These results indicated the protective effect of Vit C against DMH induced colon cancer.
Keywords
vitamin c; DMH; Colon cancer; Caspase-9 gene expression; Cytochrome 2E1; Tumor necrosis factor –alpha
Statistics
Article View: 171
PDF Download: 496