Prevalence and molecular studies on Listeria monocytogenes isolated from chicken in El-Gharbia Governorate
Abd El-Tawab, A., Homouda, S., E. El-Haw, S. (2018). Prevalence and molecular studies on Listeria monocytogenes isolated from chicken in El-Gharbia Governorate. EKB Journal Management System, 34(1), 430-442. doi: 10.21608/bvmj.2018.54506
Ashraf A Abd El-Tawab; Seham N Homouda; Shimaa E. El-Haw. "Prevalence and molecular studies on Listeria monocytogenes isolated from chicken in El-Gharbia Governorate". EKB Journal Management System, 34, 1, 2018, 430-442. doi: 10.21608/bvmj.2018.54506
Abd El-Tawab, A., Homouda, S., E. El-Haw, S. (2018). 'Prevalence and molecular studies on Listeria monocytogenes isolated from chicken in El-Gharbia Governorate', EKB Journal Management System, 34(1), pp. 430-442. doi: 10.21608/bvmj.2018.54506
Abd El-Tawab, A., Homouda, S., E. El-Haw, S. Prevalence and molecular studies on Listeria monocytogenes isolated from chicken in El-Gharbia Governorate. EKB Journal Management System, 2018; 34(1): 430-442. doi: 10.21608/bvmj.2018.54506

Prevalence and molecular studies on Listeria monocytogenes isolated from chicken in El-Gharbia Governorate

Benha Veterinary Medical Journal
Article 36, Volume 34, Issue 1, March 2018, Page 430-442  XML PDF (623.33 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.54506
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Authors
Ashraf A Abd El-Tawab1; Seham N Homouda2; Shimaa E. El-Haw3
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtoher, Benha, Egypt
2Animal Health Research Institute "Tanta branch"
3Veterinarian
Abstract
Listeria monocytogenes is the causative agent of listeriosis and food-borne disease that can lead to meningitis and bacteremia. L. monocytogenes is the most pathogenic species in the genus Listeria. A total of 400 random samples of muscles, liver, spleen and kidneys (100 samples each) were taken from 100 diseased chickens. All samples were obtained from different poultry farms and markets in El- Gharbia Governorate to estimate the prevalence of Listeria monocytogenes in chicken. The bacteriological examination of the samples resulted; 53(13.25%) isolates as 11, 21, 8 and 13 from muscles, liver, spleen and kidneys respectively. The antimicrobial sensitivity test showed that the isolated L. monocytogenes were sensitive to sulfamethoxate-trimethoprim (91.6%) followed by ampicillin, gentamycin, vancomycin and chloromphenicol (83.3%) followed by ciprofloxacin (66.6%) followed by erythromycin and tetracycline (50.0%), while the isolated strains were completely resistant to cephalothin. All L. monocytogenes strains were virulent where all of them were positive to CAMP test and Anton’s test. The PCR results for six studied strains of isolated L. monocytogenes showed that all genes (16S rRNA; inlA; prfA and hlyA) were detected (100.0%) while plcA gene was not detected. 
Keywords
Listeria monocytogenes; chicken; PCR; Virulence genes
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