The Possible Protective Effect of Propolis (Bee Glue) on Cypermethrin-Induced Hepatotoxicity in Adult Albino Rats | ||||
| Mansoura Journal of Forensic Medicine and Clinical Toxicology | ||||
| Article 2, Volume 19, Issue 1, January 2011, Page 17-32 PDF (31.39 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjfmct.2011.55319 | ||||
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| Authors | ||||
| Mie Gomaa1; Maha Abd Alla2; Maha Mohamad Sameer3 | ||||
| 1Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University,Egypt | ||||
| 2Department of Histology, Faculty of Medicine, Zagazig University, Egypt | ||||
| 3Animal Health Research Institute, Zagazig, Egypt | ||||
| Abstract | ||||
| Pyrethroid pesticides were used preferably over organochlorines and organophosphates due to their high effectiveness, low toxicity to non-target organisms and easy biodegrability. It has widespread applications in agriculture through the world resulting in increased human exposure to this compound. The aim of present study was to evaluate the possible protective effect of propolis on hepatotoxic effect caused by cypermethrin in adult male Albino rats. Fifty adult male albino rats were included in the current study and classified into 4 groups. Group I (control) subdivided into (a): negative control reclived 2ml saline arally daily and (b): positive control received orally daily 2 ml corn oil. Group II: received orally daily (cypermethrin 14.5 mg/kg) dissolved in corn oil. Group III: received orally daily (propolis 200 mg/kg) dissolved in saline. GroupIV: received orally daily (cypermethrin 14.5 mg/kg dissolved in corn oil + propolis 200 mg/kg dissolved in saline). After 4 weeks of treatment, blood samples were collected for estimation of the levels of liver enzymes [ alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP)], total proteins, albumin, total cholesterol, triglyceride, very low density lipoprotein-cholesterol (VLDL-c), then rats were sacrificed, the liver was excised, and subjected to estimation of levels of malondialdehyde (MDA), antioxidant enzymes [catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GPx)], and histopathological examination. Cypermethrin induced a significant increase in the levels of liver enzymes, total cholesterol, and MDA. While total protein, albumin, triglyceride, VLDL-c and antioxidant enzymes were decreased when compared to control rats. Histopathological examination of the liver revealed congestion of central and portal veins with hydropic degeneration of hepatocytes. Propolis administration with cypermethrin induced a significant decrease in levels of liver enzymes, total cholesterol and MDA and a significant increase in the levels of antioxidant enzymes, total protein, triglyceride and VLDL-c. Histopathological examination of the liver revealed apparent improvement of lesions induced by cypermethrin. It was concluded that propolis has a beneficial influences in reducing the hepatotoxic effects of cypermethrin in male Albino rats. | ||||
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