Using Morphological Charachters and ISJ Markers for Assessment of Genetic Diversity among Some Rice Genotypes | ||||
Alexandria Journal of Agricultural Sciences | ||||
Article 8, Volume 62, Issue 3, June 2017, Page 273-282 PDF (339.02 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/alexja.2017.5792 | ||||
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Authors | ||||
M.M. Abd El-Maksoud* 1; A.H. Abd El-Hadi2; A.B. El-Abd3; Sara El-leithy3 | ||||
1Genetics Department, Faculty of Agriculture, Mansoura University, Egypt. | ||||
2Genetics Department, Faculty of Agriculture, Mansoura University, Egypt | ||||
3Rice Research and Training Center (RRTC), Field Crop Research Institute, ARC, Egypt | ||||
Abstract | ||||
the two rice growing seasons of 2014 and 2015 at the experimental farm of Rice Research and Training Center. There were significant differences among the genotypes for all traits, indicating high genetic divergence among rice germplasm. The 24 studied genotypes were divided to 11 clusters. Cluster III included the highest number of genotypes, followed by cluster X which included four genotypes. According to the rank for desirable traits among all clusters mean values, hybridization of IR 68011-15-1-1, KATY of cluster VI with promising genotypes of cluster VII (moroberekan), cluster IX (IR 67954-46-1-3-2) and cluster XI (IR 68167-28-3-2-2, IR 69432-54-1-1-2-2 (NPT)) may be recommended as started materials for breeding program, in order to isolating transgressive segregants lines. Twelve primers of intron-exon splice junction (ISJ) were utilized in this study, out of 89 bands were detected and 44 bands showed polymorphic. The genetic similarity between genotypes ranged from 0.794 to 0.955 with an average similarity index of 0.873. Cluster analysis based on Dice's similarity coefficient using UPGMA procedure divided the studied rice genotypes into six major clusters. Furthermore, some genotypes located in one cluster alone based on either morphological or molecular dendrograms, such as genotypes number 6 (Moroberekan) and 7 (Katy). | ||||
Keywords | ||||
rice; Genetic diversity; Morphological traits; ISJ primers | ||||
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