Recent Advances in Diagnosis of Flavobacteriosis Among Some Freshwater Fishes
Eissa, I., Derwa, H., Ismaiel, M., El-Lamie, M., Elsayed, T. (2015). Recent Advances in Diagnosis of Flavobacteriosis Among Some Freshwater Fishes. EKB Journal Management System, 20(2), 31-42. doi: 10.21608/scvmj.2015.64567
Ismaiel Eissa; Hassan Derwa; Mona Ismaiel; Maather El-Lamie; T. Elsayed. "Recent Advances in Diagnosis of Flavobacteriosis Among Some Freshwater Fishes". EKB Journal Management System, 20, 2, 2015, 31-42. doi: 10.21608/scvmj.2015.64567
Eissa, I., Derwa, H., Ismaiel, M., El-Lamie, M., Elsayed, T. (2015). 'Recent Advances in Diagnosis of Flavobacteriosis Among Some Freshwater Fishes', EKB Journal Management System, 20(2), pp. 31-42. doi: 10.21608/scvmj.2015.64567
Eissa, I., Derwa, H., Ismaiel, M., El-Lamie, M., Elsayed, T. Recent Advances in Diagnosis of Flavobacteriosis Among Some Freshwater Fishes. EKB Journal Management System, 2015; 20(2): 31-42. doi: 10.21608/scvmj.2015.64567

Recent Advances in Diagnosis of Flavobacteriosis Among Some Freshwater Fishes

Suez Canal Veterinary Medical Journal. SCVMJ
Article 3, Volume 20, Issue 2, December 2015, Page 31-42  XML PDF (1.49 MB)
Document Type: Original Article
DOI: 10.21608/scvmj.2015.64567
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Authors
Ismaiel Eissa1; Hassan Derwa1; Mona Ismaiel1; Maather El-Lamie1; T. Elsayed* 2
1Dept. of Fish Diseases and Management , Faculty of Vet. Medicine, Suez Canal University
2Fish Farm Unit, Faculty of Vet. Medicine, Suez Canal University
Abstract
Columnaris disease caused by Flavobacterium columnare exists worldwide and affects a wide variety of fishes. This study aimed to investigate the isolation and characterization of Flavobacterium columnare from naturally infected fishes (Oreochromis niloticus and Clarias gariepinus) in Ismailia, using conventional biochemical methods and molecular techniques. Bacteriological examination revealed presence of 162 suspected Cytophaga isolates of which only 102 isolates were characterized as F. columnare strains according to colony character on Cytophaga agar which appeared yellow or whitish yellow in color, flat irregular with stiletto edge, clear swarming and rhizoid characters. Biochemical characterizations demonstrated that all isolates tested uniformly positive for catalase, gelatin liquefaction, absorption of Congo Red and flexirubin pigment, but they were negative for indol, Methyl red, Vogus Proskauer, and lactose, glucose, saccharose, manitol, arabinose and maltose fermentation. All isolates produced unique and clear PCR bands of the expected size (675 base pair). Consequently, PCR confirmed the positive results of the biochemical reactions of suspected isolated strain and concluded that it is of  F.columnare.
Keywords
Columnaris disease; Flavobacterium columnare; Molecular characterization; PCR; Oreochromis niloticus and Clarias gariepinus
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