Evaluation of Naturally Derived Hydroxyapatite Tissue Engineering Scaffold Coated With Chitosan-Carbon Nanotubes Composite | ||||
Egyptian Dental Journal | ||||
Article 12, Volume 65, Issue 1 - January (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics), January 2019, Page 463-474 PDF (1.6 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/edj.2019.72722 | ||||
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Authors | ||||
Salma M. Fathy1; Tarek A. Elkhooly2; Ahmed A. Emam3; Fikry M. Reicha4 | ||||
1Dental Biomaterials Dept., Faculty of Oral and Dental Medicine, Zagazig University | ||||
2Refractories, Ceramics and Building Materials Department, National Research Centre | ||||
3Medical Experimental Research Centre (MERC), Faculty of Medicine, Mansoura University | ||||
4Experimental Solid State Physics Dept., Faculty of Science, Mansoura University, Egypt | ||||
Abstract | ||||
Objective; This study aimed to valuate the effect of f-CNTs (functionalized carbon nanotubes) and chitosan (CS) composite on the mechanical properties and cell biocompatibility of carbonated HAp (hydroxyapatite) derived from cuttlefish bone. Methods; The microstructure characterization of carbonated HAp after hydrothermal treatment showed complete transformation into highly crystalline structure. The prepared CS/f-CNTs composite was introduced into HAp specimens under vacuum. A compressive load was applied till failure using a universal testing machine to evaluate compressive strength and elastic modulus of aragonite (CaCO3) and treated HAp with CS/f-CNTs composite. The proliferating number and percentage of MSCs (mesenchymal stem cells) in direct contact with tested materials were used as indication of cytotoxic effect after 24, 36 and 72 hours in addtion alkaline phosphatase activity (ALP) after 12 days was used as indication of osteogenic effect. Results; The HAp group with deposition of CS/f-CNTs composite showed a statistically significant higher compressive strength (0.82 ± 0.17 MPa) and elastic modulus (11.1 ± 1.06 MPa) and a statistically significant lower value for cell viability percentage (225,590 ± 8030.503, 32.95%) after 72 hours and ALP activity (85.51 ± 5.67 μU/μg protein) after 12 days of incubation. Conclusions; Carbonated HAp scaffolds reinforced with CS/f-CNTs composite can be used in low stress bonny defects. | ||||
Keywords | ||||
Cuttlefish; Bone scaffolds; Mechanical Properties; Cytotoxicity | ||||
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