Immunization of V-line rabbits against intestinal coccidiosis by using attenuated Eimeria oocysts.
Awade, M., Youssef, M., Alakabawy, L. (2019). Immunization of V-line rabbits against intestinal coccidiosis by using attenuated Eimeria oocysts.. EKB Journal Management System, 36(2), 314-324. doi: 10.21608/bvmj.2019.16352.1084
Marwa Awade; Mohamed Youssef; Lubna Alakabawy. "Immunization of V-line rabbits against intestinal coccidiosis by using attenuated Eimeria oocysts.". EKB Journal Management System, 36, 2, 2019, 314-324. doi: 10.21608/bvmj.2019.16352.1084
Awade, M., Youssef, M., Alakabawy, L. (2019). 'Immunization of V-line rabbits against intestinal coccidiosis by using attenuated Eimeria oocysts.', EKB Journal Management System, 36(2), pp. 314-324. doi: 10.21608/bvmj.2019.16352.1084
Awade, M., Youssef, M., Alakabawy, L. Immunization of V-line rabbits against intestinal coccidiosis by using attenuated Eimeria oocysts.. EKB Journal Management System, 2019; 36(2): 314-324. doi: 10.21608/bvmj.2019.16352.1084

Immunization of V-line rabbits against intestinal coccidiosis by using attenuated Eimeria oocysts.

Benha Veterinary Medical Journal
Article 33, Volume 36, Issue 2, June 2019, Page 314-324  XML PDF (822.89 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.16352.1084
View on SCiNiTO View on SCiNiTO
Authors
Marwa Awade email 1; Mohamed Youssef2; Lubna Alakabawy2
1Faculty of Veterinary Medicine, Benha University.
2Parasitology Dept. Fac. Vet. Med. Benha Univ.
Abstract
The present workaimed to study the immunization of V-line rabbits against coccidiosis by using attenuated Eimeria oocystsbydifferent methods (UVirradiation ,formalin and freezing-thawing ) on Eimeria oocysts and its effect on immune pattern of V-line rabbits. For this aim, Eimeria species (E. exigua,E. flavescens, Emedia, E magna, E. irresidua, E.nagpurensis, E. coecicola and E. intestinalis)were collected from infected and dead rabbits. The collected Eimeria species were sporulated in potassium dichromate 2.5% and divided into four parts ; 1stpart exposed to UV for an hour ,2nd partexposed to six freeze-thaw cycles, 3rd part was diluted with 2.5 ml of formalin 37% and 4th part left without treatment. Fifteen V-line rabbits were classified into 4 groups;G1 was treated by formalin treated Eimeria oocysts,G2 was inoculated by UV irradiated Eimeria oocysts, G3 was inoculated by freezing-thawing oocysts and G4 was inoculated by non-treated Eimeria oocysts. Challenge by non-attenuated Eimeria was carried in 14th day post inoculation.Fecal samples were collected from all groups from 6th to 35thdays, identified and count. Blood samples were collected at days (zero,7th ,14th ,21st,28th and 35th) for determination of cellular and humeral immunity. The obtained results recorded that immunization of rabbits by UV irradiated Eimeria oocysts showed low number of oocysts in feces before and after challenge compared to other groups. The same group showed high level of IgG .It recommended that immunization of rabbits by UV irradiated Eimeria oocysts could protect rabbits against coccidiosis.
Keywords
: immunization; Eimeria species; oocyst; Rabbits
Statistics
Article View: 229
PDF Download: 328