Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction
Taha, N., El barbary, H., Ibrahim, E., Mohammed, H., Wahba, N. (2019). Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction. EKB Journal Management System, 36(2), 353-359. doi: 10.21608/bvmj.2019.15171.1053
Naglaa Taha; Hend Ahmed El barbary; Ekbal Mohammed Adel Ibrahim; Hamdi Abd El- Sami Mohammed; Nahed Mohammad Mohammad Wahba. "Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction". EKB Journal Management System, 36, 2, 2019, 353-359. doi: 10.21608/bvmj.2019.15171.1053
Taha, N., El barbary, H., Ibrahim, E., Mohammed, H., Wahba, N. (2019). 'Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction', EKB Journal Management System, 36(2), pp. 353-359. doi: 10.21608/bvmj.2019.15171.1053
Taha, N., El barbary, H., Ibrahim, E., Mohammed, H., Wahba, N. Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction. EKB Journal Management System, 2019; 36(2): 353-359. doi: 10.21608/bvmj.2019.15171.1053

Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction

Benha Veterinary Medical Journal
Article 37, Volume 36, Issue 2, June 2019, Page 353-359  XML PDF (764.5 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.15171.1053
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Authors
Naglaa Taha email 1; Hend Ahmed El barbaryorcid 2; Ekbal Mohammed Adel Ibrahim3; Hamdi Abd El- Sami Mohammed3; Nahed Mohammad Mohammad Wahba4
1Food Hygiene Department, Animal Health Research Institute, Assiut. Egypt. Submitted to Faculty of Veterinary Medicine, Moshtohor, Benha University For the Degree of Ph.D (Milk Hygiene)
2Faculty of Veterinary Medicine, Benha university
3Faculty of Veterinary Medicine,Benha University
4Food Hygiene Department, Animal Health Research Institute, Assiut. Egypt
Abstract
The objectives of the current study were detection and characterization of the isolated Escherichia coli non O157 using PCR assay. A total of 90 cheese samples high salt soft cheese, Kareish cheese and Tallaga cheese were examined for E. coli non O157 using modified vancomycin- trypticase soy broth and Sorbitol MacConkey agar plates. Serodiagnosis of E. coli non-O157 has been done using slide agglutination test. Toxigenic E. coli non-O157 isolates were detected using PCR assay. Results postulated that the detection rate of non O157 using biochemical technique was 63.33% in Kariesh cheese, 20% in high salt soft cheese cheese, and 33.33% in Tallaga cheese. Ten different serotypes of E. coli non O157 have been distributed as following O1 (4.44%), O18 (1.11%), O20 (1.11%), O25 (1.11%), O26 (3.33%), O125 (1.11%), O126 (1.11%), O127 (1.11%) and untyped E. coli (18.89%). Regarding PCR results, serotypes group (O1) was positive for Stx2 gene, (O1 and O20) were positive for both Stx2 and hly genes, one serotype group O25 was positive for eaeA gene, one serotype group O26 was positive for hly gene while no detection for Stx1and STa genes. From the aforementioned data, attention must be paid to the problems of E. coli non O157 in foods. Consequently, more restriction and preventive measures should be taken in milk herds, milk production and dairy factories in respect to quality control sanitation and health care.
Keywords
E. coli non-O157; stx1; stx2; eaeA and hly genes
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