SEQUENCE ANALYSIS AND CHARACTERIZATION OF Enterococcus deurans PLASMID | ||||
| Journal of Food and Dairy Sciences | ||||
| Article 3, Volume 3, Issue 2, February 2012, Page 85-100 PDF (776.04 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jfds.2012.75306 | ||||
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| Author | ||||
| Nanis H. Gomah* | ||||
| Dairy Department, Assiut University, Assiut, Egypt | ||||
| Abstract | ||||
| More than twenty different fragments of a plasmid pNG (size 7743- 19329 bp) found in Enterococcus deurans strain resistant to different antibiotics (cefotaxime CTX, kanamycin K, methicillin MET, streptomycin S) isolated from Egyptian hard cheese were successfully cloned with the blue script vector into E- coli XL1-blue, JM 101. The Sequencing was done for all different colons. Sequencing results were subjected to Bork Group's Advanced BLAST2 search Service at EMBL for making the identification of code regions with data base similarity.The results showed that the plasmid contained different genes identified as identical to hypothetical protein gene produces "hypothetical protein" found in Entrococcus faecium plasmid pRUM, GTP-binding protein gene from Entrococcus faecium V583 produces "GTP-binding protein", V-type sodium ATP synthase subunit A ( EC 3.6.3.15) (Na(+) – translocating ATPase subunit A gene produces "Na+- ATPase alpha and beta subunit ", integral membrane protein gene:"ip-0259 " produces "integral membrane protein" from Lactobacillus Plantarum strain WCFS1 , putative RepA gene produces: " putative RepA " ;Entrococcus faecium plasmid pRUM, GTP cyclohydroase I from Lactococcus lactis subsp. Lactis (strain IL1403) , Protease gene: "sppA"; produces:"protease IV"from Shigella flexneri 2a str. 301 and other different genes. The complete sequence of the studied plasmid is now under investigation. | ||||
| Keywords | ||||
| Enteroccus deurans; plasmid; cloning; sequence analysis | ||||
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