Effect of Incubation Temperature on the Production of Fungal Cellulases | ||||
| Assiut Journal of Agricultural Sciences | ||||
| Article 9, Volume 45, Issue 2, June 2014, Page 19-29 PDF (608.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajas.2013.764 | ||||
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| Abstract | ||||
| This study was aimed to investigate the optimum incubation temperature for cellulases production by Aspergillus niger AUMC 8485, Aspergillus flavus AUMC 8534 and Emericella nidulans AUMC 8581. The obtained results showed that, the optimum incubation temperature for cellulases production as measured by cup-plate clearing zone technique was 355 C for all the studied species. The diameter of clearing zone was 22.0, 24.5, 26.0 mm for Aspergillus niger, Aspergillus flavus and Emericella nidulans, respectively. In case of exo –D-1,4 gluconase (FP-ase) production, the highest enzymatic activity was obtained at 355 C by all the studied fungal species. Meanwhile, the highest specific activity was recorded at 405 C for A. niger and at 355 C for A. flavus and E. nidulans. At the same time, the optimum incubation temperature for endo- ß- D-1,4 gluconase (CMC-ase) production was recorded at 405 C for A. niger and E. nidulans, and at 35 5 C for A. flavus. The highest CMC-ase specific activity was observed at 405 C for all the studied strains. The maximum amount of reducing sugars found in the culture filtrate at 355 C for A. niger, and at 405 C for A. flavus and E. nidulans. Also, the highest amount of soluble protein was recorded at 355 C for A. niger and at 305 C for A. flavus and E. nidulans | ||||
| Keywords | ||||
| Cellulases; Temperature; Aspergillus niger; Aspergillus flavus; Emericella nidulans | ||||
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