In vitro characterization and pathogenesis of Egypt/Beni-Suef; a novel genotype of infectious bronchitis virus
Abdel-Moneim, A., Madbouly, H., El-Kady, M. (2005). In vitro characterization and pathogenesis of Egypt/Beni-Suef; a novel genotype of infectious bronchitis virus. EKB Journal Management System, 15(2), 127-133. doi: 10.21608/jvmr.2005.77943
A. S. Abdel-Moneim; H. M. Madbouly; M. F. El-Kady. "In vitro characterization and pathogenesis of Egypt/Beni-Suef; a novel genotype of infectious bronchitis virus". EKB Journal Management System, 15, 2, 2005, 127-133. doi: 10.21608/jvmr.2005.77943
Abdel-Moneim, A., Madbouly, H., El-Kady, M. (2005). 'In vitro characterization and pathogenesis of Egypt/Beni-Suef; a novel genotype of infectious bronchitis virus', EKB Journal Management System, 15(2), pp. 127-133. doi: 10.21608/jvmr.2005.77943
Abdel-Moneim, A., Madbouly, H., El-Kady, M. In vitro characterization and pathogenesis of Egypt/Beni-Suef; a novel genotype of infectious bronchitis virus. EKB Journal Management System, 2005; 15(2): 127-133. doi: 10.21608/jvmr.2005.77943

In vitro characterization and pathogenesis of Egypt/Beni-Suef; a novel genotype of infectious bronchitis virus

Journal of Veterinary Medical Research
Article 20, Volume 15, Issue 2, March 2005, Page 127-133  XML PDF (465.18 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2005.77943
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Authors
A. S. Abdel-Moneim1; H. M. Madbouly2; M. F. El-Kady3
1Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
2Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
3Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
Abstract
In vitro characterizations of infectious bronchitis virus (Egypt/Beni-Suef/01 strain) were studied. Heat stability at 56 °C for 5, 10, 15, 20, and 30 min, UV irradiation for 10, 15, 20, and 30 min, chloroform sensitivity, pH stability, and E/M examination were screened. Two-week-old commercial chickens were inoculated
intraocularly with Egypt/Beni-Suef/01. At days 1, 3, 5, 7, 11 and 14 days postinfection, three birds were euthanatized, and virus antigens were screened using
immunofluorescence. Histopathology of both trachea & kidney was also performed.
Egypt /Beni-Suef/01 was found to be sensitive to 56 °C for 5 min, UV for 10 min
and chloroform but resistant to pH 3 and pH 9. It showed typical morphology of
coronaviruses by E/M. The virus was found to induce mild respiratory signs and
sever renal changes. Immunoflourescence staining showed viral antigen in larynx,
trachea, thymus, lung, liver, spleen, renal tubules and glomeruli as well as bursa of
Fabricius but not in pancreas. Marked histopathological lesions were also detected
in both trachea and kidneys. 
Keywords
Vitro; Characterization; pathogenesis; genotype; infectious; bronchitis; virus
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