Distribution of potentially pathogenic Acanthamoeba isolates in the environment of Helwan University, Egypt
Koteit, H., E. Elowa, S., Z. Al-Herrawy, A. (2020). Distribution of potentially pathogenic Acanthamoeba isolates in the environment of Helwan University, Egypt. EKB Journal Management System, 24(3), 61-73. doi: 10.21608/ejabf.2020.87814
Heba Koteit; Shehata E. Elowa; Ahmad Z. Al-Herrawy. "Distribution of potentially pathogenic Acanthamoeba isolates in the environment of Helwan University, Egypt". EKB Journal Management System, 24, 3, 2020, 61-73. doi: 10.21608/ejabf.2020.87814
Koteit, H., E. Elowa, S., Z. Al-Herrawy, A. (2020). 'Distribution of potentially pathogenic Acanthamoeba isolates in the environment of Helwan University, Egypt', EKB Journal Management System, 24(3), pp. 61-73. doi: 10.21608/ejabf.2020.87814
Koteit, H., E. Elowa, S., Z. Al-Herrawy, A. Distribution of potentially pathogenic Acanthamoeba isolates in the environment of Helwan University, Egypt. EKB Journal Management System, 2020; 24(3): 61-73. doi: 10.21608/ejabf.2020.87814

Distribution of potentially pathogenic Acanthamoeba isolates in the environment of Helwan University, Egypt

Egyptian Journal of Aquatic Biology and Fisheries
Article 6, Volume 24, Issue 3, May and June 2020, Page 61-73  XML PDF (860.93 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2020.87814
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Authors
Heba Koteit; Shehata E. Elowa; Ahmad Z. Al-Herrawy
Abstract
Acanthamoeba species are free-living amoebae having worldwide distribution. These amoebae can cause granulomatous amoebic encephalitis and amoebic keratitis in humans. They can produce proteases that are considered virulence factors. Acanthamoeba can also harbor pathogenic bacteria, fungi, and viruses.
The objective of this study is to evaluate the presence of Acanthamoeba in the environment of Helwan University, Egypt.
Six types of samples (tap water, irrigation water, wastewater, swabs from surfaces, soil, and air) were collected, processed, and cultured on non-nutrient agar medium. Positive plates for Acanthamoeba were subcultured, purified and amoebae were identified morphologically and confirmed by PCR using Acanthamoeba genus-specific primers.
Obtained results declared that members of genus Acanthamoeba were detected in 91.7, 83.3, 54.2, 45.8, 12.5 and 12.5% of irrigation water, soil, swabs, wastewater, tap water, and air samples, respectively. The morphologically identified Acanthamoeba species proved to be related to genus Acanthamoeba when tested by PCR. Statistically, the sampling source had a strong significant correlation with the prevalence of Acanthamoeba. The highest appearance of Acanthamoeba was recorded in the spring season for samples from irrigation water, soil, and swabs from surfaces.
In conclusion, the high prevalence of Acanthamoeba species in irrigation water and soil exert public health hazards to students and workers in Helwan University. 
Keywords
Acanthamoeba; Environment; Helwan University; Egypt
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