Biochemical and histopathological effect of probiotics on experimentallyinduced liver fibrosis in rat | ||||
| Benha Veterinary Medical Journal | ||||
| Article 35, Volume 31, Issue 2, December 2016, Page 248-253 PDF (474.21 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31306 | ||||
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| Authors | ||||
| Mahfouz MK1; Omnia M. Abd El-Hamid1; Emam MA2; Menna. A. Bakr1 | ||||
| 1Biochemistry and Clinical Biochemistry Department, Faculty of Veterinary Medicine, Benha University | ||||
| 2Histology and Cytology Department, Faculty of Veterinary Medicine, Benha University, 13736 Egypt | ||||
| Abstract | ||||
| The objective of the present study was to evaluate the biochemical effect of probiotics treatment on Thioacetamide (TAA) induced liver fibrosis in rats. Thirty white male Sprague Dewily rats of 8-10 weeks old and Weighing 150 to 200 g were used in the experiment. Rats were randomly divided in to 2 main groups, 1st group acts as normal control, 2nd group was injected with TAA intraperitoneally (200mg/kg b.wt) twice a week for 6 weeks for the induction of liver fibrosis, after that the 2nd group was subdivided into 2 subgroups1st subgroup received no drugs and served as positive control, 2nd subgroup was treated with a daily dose of probiotics (0.0128×109 bacteria per gram of rat body weight) which is equivalent to (135mg/kgb.wt) orally for 6 weeks. Blood samples were collected twice after 3 and 6 weeks of probiotics treatment for biochemical examination, then rats were decapitated and liver specimens were collected for histopathological examination. Intraperitoneal supplementation of TAA caused increase in serum level of AST, ALT, Tbil, Dbil and pro-inflammatory cytokines as TNFα and IL6, decrease in serum level of Albumin and total protein. Furthermore, histopathological examination revealed extensive degeneration, inflammatory infiltration and nodulation of the liver. Probiotics treatment revealed decrease in serum level of ALT, AST, Tbil, Dbil and pro-inflammatory cytokines as TNFα and IL6, decrease in serum level of Albumin and Total protein. Moreover, histopathological examination revealed mild inflammation and less nodulation of the hepatic parenchyma. | ||||
| Keywords | ||||
| TAA; probiotics; TNFα; IL6; Liver fibrosis | ||||
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